QTL analysis for nitrogen use efficiency in wheat (Triticum aestivum L.)

The genetic architecture of nitrogen use efficiency (NUE) and its two component traits i.e. NUpE (N uptake efficiency) NUtE utilization was studied using a bi-parental RIL mapping population derived from cross HUW468 (high NUE)/C306 (low NUE). population, parental genotypes three check were evaluated under four different N levels (0, 60, 120, 180 kg/ha) over years. A map containing 456 SNP markers (2571.38 cM length) used for QTL analysis. Thirty six main effect QTLs (17 NUE, 13 6 NUtE) distributed on 12 chromosomes (1B, 1D, 2A, 2B, 3A, 4B, 5A, 5B, 5D, 6A, 6D, 7A) identified at 2.52–9.27 LOD scores. Individual explained 6.65–22.89% phenotypic variation. Multi-traits (Mt-QTLs) epistatic involving first-order (QTL × QTL) interactions also discovered. Candidate genes (CGs, as many 737) mined regions which mainly involved in metabolic process, cellular process catalytic activity, etc.; differential expression observed 49 CGs roots 34 shoots. encoded important transcription factors, transporters, etc. having role NUE. reported this study enriched the available knowledge. Seven (including Mt-QTLs) are recommended MAS improvement NUE wheat.